Apoptosis in the Medaka Embryo in the Early Developmental Stage

Apoptosis is an important event of the development of various organs. In this study, we used in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) to visualize the temporal and spatial distribution of apoptosis in the developing medaka embryo, which is a useful model for developmental biology and genetics. Most of the apoptotic cells were distributed in the central nervous system and tailbud. In the brain and retina, most of the apoptosis occurred in the restricted period. In situ hybridization against caspase 3A and caspase 3B showed that these were distributed in the tailbud and the head, respectively. These results suggested that two types of caspase 3 were involved in apoptosis in different areas

Exergy analysis:Tthe effect of relative humidity, air temperature and effective clothing insulation on thermal comfort

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Nucleation and Bulk Crystallization in Binary Phase Field Theory

We present a phase field theory for binary crystal nucleation. In the one-component limit, quantitative agreement is achieved with computer simulations (Lennard-Jones system) and experiments (ice-water system) using model parameters evaluated from the free energy and thickness of the interface. The critical undercoolings predicted for Cu-Ni alloys accord with the measurements, and indicate homogeneous nucleation. The Kolmogorov exponents deduced for dendritic solidification and for "soft-impingement" of particles via diffusion fields are consistent with experiment.Comment: 4 pages, 4 figures, accepted to PR

V5852 Sgr : an unusual nova possibly associated with the Sagittarius stream

We report spectroscopic and photometric follow-up of the peculiar nova V5852~Sgr (discovered as OGLE-2015-NOVA-01), which exhibits a combination of features from different nova classes. The photometry shows a flat-topped light curve with quasi-periodic oscillations, then a smooth decline followed by two fainter recoveries in brightness. Spectroscopy with the Southern African Large Telescope shows first a classical nova with an Fe II or Fe IIb spectral type. In the later spectrum, broad emissions from helium, nitrogen and oxygen are prominent and the iron has faded which could be an indication to the start of the nebular phase. The line widths suggest ejection velocities around 1000 km s-1. The nova is in the direction of the Galactic bulge and is heavily reddened by an uncertain amount. The V magnitude 16 days after maximum enables a distance to be estimated and this suggests that the nova may be in the extreme trailing stream of the Sagittarius dwarf spheroidal galaxy. If so it is the first nova to be detected from that, or from any dwarf spheroidal galaxy. Given the uncertainty of the method and the unusual light curve we cannot rule out the possibility that it is in the bulge or even the Galactic disk behind the bulge.Publisher PDFPeer reviewe

A New SU UMa-Type Dwarf Nova, QW Serpentis (= TmzV46)

We report on the results of the QW Ser campaign which has been continued from 2000 to 2003 by the VSNET collaboration team. Four long outbursts and many short ones were caught during this period. Our intensive photometric observations revealed superhumps with a period of 0.07700(4) d during all four superoutbursts, proving the SU UMa nature of this star. The recurrence cycles of the normal outbursts and the superoutbursts were measured to be $\sim$50 days and 240(30) days, respectively. The change rate of the superhump period was -5.8x10^{-5}. The distance and the X-ray luminosity in the range of 0.5-2.4 keV are estimated to be 380(60) pc and log L_x = 31.0 \pm 0.1 erg s^{-1}. These properties have typical values for an SU UMa-type dwarf nova with this superhump period.Comment: 9 pages, 12 figures, to appear in the VSNET special issue of PAS

Synthetic long non-coding RNAs [SINEUPs] rescue defective gene expression in vivo

Non-coding RNAs provide additional regulatory layers to gene expression as well as the potential to being exploited as therapeutic tools. Non-coding RNA-based therapeutic approaches have been attempted in dominant diseases, however their use for treatment of genetic diseases caused by insufficient gene dosage is currently more challenging. SINEUPs are long antisense non-coding RNAs that up-regulate translation in mammalian cells in a gene-specific manner, although, so far evidence of SINEUP efficacy has only been demonstrated in in vitro systems. We now show that synthetic SINEUPs effectively and specifically increase protein levels of a gene of interest in vivo. We demonstrated that SINEUPs rescue haploinsufficient gene dosage in a medakafish model of a human disorder leading to amelioration of the disease phenotype. Our results demonstrate that SINEUPs act through mechanisms conserved among vertebrates and that SINEUP technology can be successfully applied in vivo as a new research and therapeutic tool for gene-specific up-regulation of endogenous functional proteins

The Cyprinodon variegatus genome reveals gene expression changes underlying differences in skull morphology among closely related species

Genes in durophage intersection set at 15 dpf. This is a comma separated table of the genes in the 15 dpf durophage intersection set. Given are edgeR results for each pairwise comparison. Columns indicating whether a gene is included in the intersection set at a threshold of 1.5 or 2 fold are provided. (CSV 13 kb

Surface Structure of Liquid Metals and the Effect of Capillary Waves: X-ray Studies on Liquid Indium

We report x-ray reflectivity (XR) and small angle off-specular diffuse scattering (DS) measurements from the surface of liquid Indium close to its melting point of $156^\circ$C. From the XR measurements we extract the surface structure factor convolved with fluctuations in the height of the liquid surface. We present a model to describe DS that takes into account the surface structure factor, thermally excited capillary waves and the experimental resolution. The experimentally determined DS follows this model with no adjustable parameters, allowing the surface structure factor to be deconvolved from the thermally excited height fluctuations. The resulting local electron density profile displays exponentially decaying surface induced layering similar to that previously reported for Ga and Hg. We compare the details of the local electron density profiles of liquid In, which is a nearly free electron metal, and liquid Ga, which is considerably more covalent and shows directional bonding in the melt. The oscillatory density profiles have comparable amplitudes in both metals, but surface layering decays over a length scale of $3.5\pm 0.6$ \AA for In and $5.5\pm 0.4$ \AA for Ga. Upon controlled exposure to oxygen, no oxide monolayer is formed on the liquid In surface, unlike the passivating film formed on liquid Gallium.Comment: 9 pages, 5 figures; submitted to Phys. Rev.

Monte Carlo Methods for Estimating Interfacial Free Energies and Line Tensions

Excess contributions to the free energy due to interfaces occur for many problems encountered in the statistical physics of condensed matter when coexistence between different phases is possible (e.g. wetting phenomena, nucleation, crystal growth, etc.). This article reviews two methods to estimate both interfacial free energies and line tensions by Monte Carlo simulations of simple models, (e.g. the Ising model, a symmetrical binary Lennard-Jones fluid exhibiting a miscibility gap, and a simple Lennard-Jones fluid). One method is based on thermodynamic integration. This method is useful to study flat and inclined interfaces for Ising lattices, allowing also the estimation of line tensions of three-phase contact lines, when the interfaces meet walls (where "surface fields" may act). A generalization to off-lattice systems is described as well. The second method is based on the sampling of the order parameter distribution of the system throughout the two-phase coexistence region of the model. Both the interface free energies of flat interfaces and of (spherical or cylindrical) droplets (or bubbles) can be estimated, including also systems with walls, where sphere-cap shaped wall-attached droplets occur. The curvature-dependence of the interfacial free energy is discussed, and estimates for the line tensions are compared to results from the thermodynamic integration method. Basic limitations of all these methods are critically discussed, and an outlook on other approaches is given